Ιδρυματικό Αποθετήριο
Πολυτεχνείο Κρήτης
Πολυτεχνείο Κρήτης
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| URI | http://purl.tuc.gr/dl/dias/1591F8D8-F4AC-4A92-BAD2-6F0E8DE620D5 | - |
| Αναγνωριστικό | http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.2000.00797.x/abstract | - |
| Αναγνωριστικό | https://doi.org/ 10.1046/j.1365-2672.2000.00797.x | - |
| Γλώσσα | en | - |
| Μέγεθος | 5 pages | en |
| Τίτλος | Development of a multiplex PCR detection of Salmonella spp. and Shigella spp. in mussels | en |
| Δημιουργός | Vantarakis, Apostolos | en |
| Δημιουργός | Komninou, Georgia | en |
| Δημιουργός | Venieri Danai | en |
| Δημιουργός | Βενιερη Δαναη | el |
| Δημιουργός | Papapetropoulou Maria | en |
| Εκδότης | The Society for Applied Microbiology | en |
| Περίληψη | Multiplex PCR amplification of invA and virA genes was developed enabling simultaneous detection in mussels of Salmonella spp. and Shigella spp., respectively. Simultaneous amplification of products of 215 and 275 bp was obtained either by using mixtures of individual strains of Sh. dysenteriae and Salm. typhimurium or spiked contaminated mussels with both bacteria. In the case of the mussels, 10-100 cells of Salmonella spp. and Shigella per millilitre of homogenate were detected by the multiplex PCR following a pre-enrichment step to increase sensitivity and to ensure that detection was based on the presence of cultivable bacteria. Also, the sensitivity and specificity of this method was evaluated. Multiplex PCR amplification was shown to be an effective, sensitive and rapid method for the simultaneous detection of pathogens in mussels. | en |
| Τύπος | Peer-Reviewed Journal Publication | en |
| Τύπος | Δημοσίευση σε Περιοδικό με Κριτές | el |
| Άδεια Χρήσης | http://creativecommons.org/licenses/by/4.0/ | en |
| Ημερομηνία | 2015-11-03 | - |
| Ημερομηνία Δημοσίευσης | 2000 | - |
| Θεματική Κατηγορία | PCR | en |
| Βιβλιογραφική Αναφορά | A. Vantarakis, G. Komninou, D. Venieri and M. Papapetropoulou, "Development of a multiplex PCR detection of salmonella spp. and shigella spp. in mussels", Lett. Appl. Microbiol., vol. 31, no. 2, pp. 105-109, Sep. 2000. doi:10.1046/j.1365-2672.2000.00797.x | en |
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