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Proteome changes during transition from human embryonic to vascular progenitor cells

Tsolis Konstantinos C., Bagli Eleni, Kanaki Katerina, Zografou Sofia, Carpentier Sébastien Christian, Bei Aikaterini, Christoforidis Savvas, Zervakis Michail, Murphy Carol, Fotsis Theodore, Economou, Anastassios

Απλή Εγγραφή


URIhttp://purl.tuc.gr/dl/dias/89FC39C3-64A9-4BF5-9EEE-9A18E29468F4-
Αναγνωριστικόhttps://pubs.acs.org/doi/10.1021/acs.jproteome.6b00180-
Αναγνωριστικόhttps://doi.org/10.1021/acs.jproteome.6b00180-
Γλώσσαen-
Μέγεθος13 pagesen
ΤίτλοςProteome changes during transition from human embryonic to vascular progenitor cellsen
ΔημιουργόςTsolis Konstantinos C.en
ΔημιουργόςBagli Elenien
ΔημιουργόςKanaki Katerinaen
ΔημιουργόςZografou Sofiaen
ΔημιουργόςCarpentier Sébastien Christianen
ΔημιουργόςBei Aikaterinien
ΔημιουργόςΜπεη Αικατερινηel
ΔημιουργόςChristoforidis Savvasen
ΔημιουργόςZervakis Michailen
ΔημιουργόςΖερβακης Μιχαηλel
ΔημιουργόςMurphy Carolen
ΔημιουργόςFotsis Theodoreen
ΔημιουργόςEconomou, Anastassiosen
ΕκδότηςAmerican Chemical Societyen
ΠερίληψηHuman embryonic stem cells (hESCs) are promising in regenerative medicine (RM) due to their differentiation plasticity and proliferation potential. However, a major challenge in RM is the generation of a vascular system to support nutrient flow to newly synthesized tissues. Here we refined an existing method to generate tight vessels by differentiating hESCs in CD34+ vascular progenitor cells using chemically defined media and growth conditions. We selectively purified these cells from CD34- outgrowth populations also formed. To analyze these differentiation processes, we compared the proteomes of the hESCs with those of the CD34+ and CD34- populations using high resolution mass spectrometry, label-free quantification, and multivariate analysis. Eighteen protein markers validate the differentiated phenotypes in immunological assays; nine of these were also detected by proteomics and show statistically significant differential abundance. Another 225 proteins show differential abundance between the three cell types. Sixty-three of these have known functions in CD34+ and CD34- cells. CD34+ cells synthesize proteins implicated in endothelial cell differentiation and smooth muscle formation, which support the bipotent phenotype of these progenitor cells. CD34- cells are more heterogeneous synthesizing muscular/osteogenic/chondrogenic/adipogenic lineage markers. The remaining >150 differentially abundant proteins in CD34+ or CD34- cells raise testable hypotheses for future studies to probe vascular morphogenesis.en
ΤύποςPeer-Reviewed Journal Publicationen
ΤύποςΔημοσίευση σε Περιοδικό με Κριτέςel
Άδεια Χρήσηςhttp://creativecommons.org/licenses/by/4.0/en
Ημερομηνία2018-10-11-
Ημερομηνία Δημοσίευσης2016-
Θεματική ΚατηγορίαAngiogenesisen
Θεματική ΚατηγορίαCD34+en
Θεματική ΚατηγορίαComparative proteomicsen
Θεματική ΚατηγορίαEndothelial progenitor cellsen
Θεματική ΚατηγορίαhESCsen
Θεματική ΚατηγορίαMultivariate analysisen
Θεματική ΚατηγορίαPLSen
Θεματική ΚατηγορίαVascular cell differentiationen
Βιβλιογραφική ΑναφοράK. C. Tsolis, E. Bagli, K. Kanaki, S. Zografou, S. Carpentier, E. S. Bei, S. Christoforidis, M. Zervakis, C. Murphy, T. Fotsis and A. Economou, "Proteome changes during transition from human embryonic to vascular progenitor cells," J. Proteome Res., vol. 15, no. 6, pp. 1995-2007, Jun. 2016. doi: 10.1021/acs.jproteome.6b00180en

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