Ιδρυματικό Αποθετήριο
Πολυτεχνείο Κρήτης
Πολυτεχνείο Κρήτης
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| URI | http://purl.tuc.gr/dl/dias/A9DFD49C-5659-4A36-94B9-E50787C717C4 | - |
| Αναγνωριστικό | https://doi.org/10.1177/1758835919895754 | - |
| Αναγνωριστικό | https://journals.sagepub.com/doi/full/10.1177/1758835919895754 | - |
| Γλώσσα | en | - |
| Μέγεθος | 12 pages | en |
| Μέγεθος | 769,01 kilobytes | en |
| Τίτλος | CXCR4 and JUNB double-positive disseminated tumor cells are detected frequently in breast cancer patients at primary diagnosis | en |
| Δημιουργός | Kallergi Galatea | en |
| Δημιουργός | Hoffmann Oliver | en |
| Δημιουργός | Bittner Ann-Kathrin | en |
| Δημιουργός | Papadimitriou Lina | en |
| Δημιουργός | Katsarou Spyridoula D. | en |
| Δημιουργός | Zacharopoulou Nefeli | en |
| Δημιουργός | Zervakis Michail | en |
| Δημιουργός | Ζερβακης Μιχαηλ | el |
| Δημιουργός | Sfakianakis Stelios | en |
| Δημιουργός | Stournaras Christos | en |
| Δημιουργός | Georgoulias Vassilis | en |
| Δημιουργός | Kimmig, Rainer | en |
| Δημιουργός | Kasimir-Bauer, Sabine, 1963- | en |
| Εκδότης | SAGE Publications | en |
| Περίληψη | Background: The chemokine receptor CXCR4 and the transcription factor JUNB, expressed on a variety of tumor cells, seem to play an important role in the metastatic process. Since disseminated tumor cells (DTCs) in the bone marrow (BM) have been associated with worse outcomes, we evaluated the expression of CXCR4 and JUNB in DTCs of primary, nonmetastatic breast cancer (BC) patients before the onset of any systemic treatment. Methods: Bilateral BM (10 ml) aspirations of 39 hormone receptor (HR)-positive, HER2-negative BC patients were assessed for the presence of DTCs using the following combination of antibodies: pan-cytokeratin (A45-B/B3)/CXCR4/JUNB. An expression pattern of the examined proteins was created using confocal laser scanning microscopy, Image J software and BC cell lines. Results: CXCR4 was overexpressed in cancer cells and DTCs, with the following hierarchy of expression: SKBR3 > MCF7 > DTCs > MDA-MB231. Accordingly, the expression pattern of JUNB was: DTCs > MDA-MB231 > SKBR3 > MCF7. The mean intensity of CXCR4 (6411 ± 334) and JUNB (27725.64 ± 470) in DTCs was statistically higher compared with BM hematopoietic cells (2009 ± 456, p = 0.001; and 11112.89 ± 545, p = 0.001, respectively). The (CXCR4+JUNB+CK+) phenotype was the most frequently detected [90% (35/39)], followed by the (CXCR4–JUNB+CK+) phenotype [36% (14/39)]. However, (CXCR4+JUNB–CK+) tumor cells were found in only 5% (3/39) of patients. Those patients harboring DTCs with the (CXCR4+JUNB+CK+) phenotype revealed lower overall survival (Cox regression: p = 0.023). Conclusions: (CXCR4+JUNB+CK+)-expressing DTCs, detected frequently in the BM of BC patients, seem to identify a subgroup of patients at higher risk for relapse that may be considered for close follow up. | en |
| Τύπος | Peer-Reviewed Journal Publication | en |
| Τύπος | Δημοσίευση σε Περιοδικό με Κριτές | el |
| Άδεια Χρήσης | http://creativecommons.org/licenses/by-nc/4.0/ | en |
| Ημερομηνία | 2021-09-07 | - |
| Ημερομηνία Δημοσίευσης | 2020 | - |
| Θεματική Κατηγορία | CXCR4 | en |
| Θεματική Κατηγορία | Disseminated tumor cells | en |
| Θεματική Κατηγορία | Dormancy | en |
| Θεματική Κατηγορία | JUNB | en |
| Θεματική Κατηγορία | Primary breast cancer | en |
| Βιβλιογραφική Αναφορά | G. Kallergi, O. Hoffmann, A.-K. Bittner, L. Papadimitriou, S. D. Katsarou, N. Zacharopoulou, M. Zervakis, S. Sfakianakis, C. Stournaras, V. Georgoulias, R. Kimmig, and S. Kasimir-Bauer, “CXCR4 and JUNB double-positive disseminated tumor cells are detected frequently in breast cancer patients at primary diagnosis,” Ther. Adv. Med. Oncol., vol. 12, Jan. 2020. doi: 10.1177/1758835919895754 | en |
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