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Commitment of CO2 from power plant waste gas by culturing the microalgae Stichococcus sp.

Mitrogiannis Dimitrios

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Summary

The research purpose was the reduction of CO2 from flue gases with the use of microalgae and bioconversion of biomass into high-value products. The present study took place in the thermal power plants AIS Kerateas-Lavriou of the Public Power Corporation (PPC). 15 L closed type photo-bioreactors were utilized for microalgae cultivation, in which the cells were cultivated attached on sandblasted glass. The lab photo-bioreactor was placed inside a small shelter providing protection from weather conditions and other limiting factors. Stichococcus sp. wasused throughout the experiments. Carbon under the form of carbon dioxide contained in flue gas was provided into the photo-bioreactor at a rate of 0,6 L per minute. Three types of cultures were examined: cultivation with continuous light, with alternating light and with nitrogen deficiency. Cultures were illuminated by LED lamps (6.600, 6.600 and 3.300 lux for cultivation with continuous light, alternating light and with nitrogen deficiency, respectively) and the ambient temperature was controlled by an A/C unit (25 ± 1 oC). Typically, Stichococcus sp. was cultivated in artificial seawater with the addition of Bold’s Basal Medium. The composition ofNaCl and NaNO3 was 35 g/L and 0.75 g/L, respectively. Also, nitrogen starvation was implemented 3 days prior to biomass harvesting, in order to increase lipids production. At the end of cultivation period, the extracted biomass was converted into high added value products (i.e., lipids, pigments, proteins and carbohydrates), to assess Stichococcus sp. potential for 3rd generation biorefineries.The results of Stichococcus sp. cultivation for biomass production were 50,5, 47,9 and 38,3 g/m2 for cultivation with continuous light, alternating light and with nitrogen deficiency respectively. The bioproducts content of Stichococcus sp. was approximately 80-90%, the bioproducts include carbohydrates, lipids proteins and total chlorophyll. Carbohydrates content was the highest amongst other bio-products, being 24,8, 23,4 and 11,9 g/m2 respectively. The lipid content was 6,6, 6,8 and 11 g/m2. The proteins were 8,3, 7,6 and 6,1 g/m2 and the totalchlorophyll found to be 19,1∙10-2, 23∙10-2, 12∙10-2 g/m2.

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