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Determination of enterobacteria in air and wastewater samples from a wastewater treatment plant by epi-fluorescence microscopy

N. Patentalakis, Pantidou Ariadni, Kalogerakis Nikos

Απλή Εγγραφή


URIhttp://purl.tuc.gr/dl/dias/804B31EF-07F0-4ECC-9E56-465776355002-
Αναγνωριστικόhttps://doi.org/10.1007/s11267-007-9135-9-
Μέγεθος8en
ΤίτλοςDetermination of enterobacteria in air and wastewater samples from a wastewater treatment plant by epi-fluorescence microscopyen
ΔημιουργόςN. Patentalakisen
ΔημιουργόςPantidou Ariadnien
ΔημιουργόςΠαντιδου Αριαδνηel
ΔημιουργόςKalogerakis Nikosen
ΔημιουργόςΚαλογερακης Νικοςel
ΠεριγραφήΔημοσίευση σε επιστημονικό περιοδικό el
ΠερίληψηBacteria, fungi and viruses are often encountered in aerosols and they can be pathogenic or cause allergies following inhalation. Wastewater treatment facilities have been found to generate bioaerosols, which are transported by the prevailing winds downstream to areas that can be up to several hundred meters away. Bioaerosol formation has a significant effect on air quality in the vicinity of the treatment plants. The amount and characteristics of the formed bioaerosols depend on the aeration system employed at the aeration tank of the wastewater treatment facility. In this work we determined Enterobacteria in air and wastewater samples at the main wastewater treatment facility of the city of Chania (Crete, Greece). Concentrations of airborne bacteria were measured near the aeration and sedimentation tanks. Samples of airborne bacteria were taken by using Merck’s MAS-100 bioaerosol collector followed by incubation and enumeration of the colonies. The use of different growth media enabled the separation and enumeration of several classes of microorganisms. As part of this study, Enterobacteria in air samples were also determined by filtration sampling followed by analysis of the collected microorganisms using DAPI staining to determine total cell counts (both viable and non-viable cells). Fluorescence in situ hybridization (FISH) with specific 23S rRNA probes was also used in order to identify specific groups of microorganisms (well known pathogens) present in the bioaerosols. The analysis was also performed in wastewater taken from the aeration and secondary sedimentation tanks in an effort to correlate the airborne bacteria with those in the wastewater.en
ΤύποςPeer-Reviewed Journal Publicationen
ΤύποςΔημοσίευση σε Περιοδικό με Κριτέςel
Άδεια Χρήσηςhttp://creativecommons.org/licenses/by/4.0/en
Ημερομηνία2015-10-15-
Ημερομηνία Δημοσίευσης2007-
Θεματική ΚατηγορίαEHEC (Bacterium)en
Θεματική ΚατηγορίαEnterohemorrhagic Escherichia colien
Θεματική ΚατηγορίαEscherichia coli O157en
Θεματική Κατηγορίαescherichia coli o157 h7en
Θεματική Κατηγορίαehec bacteriumen
Θεματική Κατηγορίαenterohemorrhagic escherichia colien
Θεματική Κατηγορίαescherichia coli o157en
Θεματική ΚατηγορίαKlebsiella pneumoniaeen
Θεματική ΚατηγορίαDAPIen
Θεματική ΚατηγορίαFISHen
Θεματική ΚατηγορίαWastewater treatment plant en
Θεματική ΚατηγορίαBioaerosolen
Βιβλιογραφική ΑναφοράN. Patentalakis, A. Pantidou and N. Kalogerakis, "Determination of enterobacteria in air and wastewater samples from a wastewater treatment plant by epi-fluorescence microscopy," Water Air Soil Pollution: Focus, 8, vol. 8, no. 1, pp. 107-115, Feb. 2008. doi: 10.1007/s11267-007-9135-9en

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