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Automated analysis of fish and immunohistochemistry images: A review

Zervakis Michalis, Pitas, Ioannis, Zenonas Theodosiou, Kleoniki Lyroudia

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URIhttp://purl.tuc.gr/dl/dias/6B3B0DD1-6594-4743-B155-4FE9EE826E56-
Αναγνωριστικόhttps://doi.org/10.1002/cyto.a.20409-
Γλώσσαen-
Μέγεθος12 pagesen
ΤίτλοςAutomated analysis of fish and immunohistochemistry images: A reviewen
ΔημιουργόςZervakis Michalisen
ΔημιουργόςΖερβακης Μιχαληςel
ΔημιουργόςPitas, Ioannisen
ΔημιουργόςZenonas Theodosiouen
ΔημιουργόςKleoniki Lyroudiaen
ΕκδότηςInternational Society for Analytical Cytologyen
ΠερίληψηFluorescent in-situ hybridization (FISH) and immunohistochemistry (IHC) constitute a pair of complimentary techniques for detecting gene amplification and overexpression, respectively. The advantages of IHC include relatively cheap materials and high sample durability, while FISH is the more accurate and reproducible method. Evaluation of FISH and IHC images is still largely performed manually, with automated or semiautomated techniques increasing in popularity. Here, we provide a comprehensive review of a number of (semi-) automated FISH and IHC image processing systems, focusing on the algorithmic aspects of each technique. Our review verifies the increasingly important role of such methods in FISH and IHC; however, manual intervention is still necessary in order to resolve particularly challenging or ambiguous cases. In addition, large-scale validation is required in order for these systems to enter standard clinical practiceen
ΤύποςPeer-Reviewed Journal Publicationen
ΤύποςΔημοσίευση σε Περιοδικό με Κριτέςel
Άδεια Χρήσηςhttp://creativecommons.org/licenses/by/4.0/en
Ημερομηνία2015-10-23-
Ημερομηνία Δημοσίευσης2007-
Βιβλιογραφική ΑναφοράZ. Theodosiou, I.N. Kasampalidis, G. Livanos, M. Zervakis, I. Pitas, K. Lyroudia," Automated analysis of FISH and immunohistochemistry images: a review ,"Cytometry Part A,vol. 71,no.7 , pp. 439-450.2007.doi:10.1002/cyto.a.20409en

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