Ιδρυματικό Αποθετήριο
Πολυτεχνείο Κρήτης
Πολυτεχνείο Κρήτης
EN
|
EL
| URI | http://purl.tuc.gr/dl/dias/A807AD7B-4F26-4A45-B27C-46C8B54C5CFF | - |
| Αναγνωριστικό | http://thales.iacm.forth.gr/~emilona/MylonaESBMEProceedings08.pdf | - |
| Γλώσσα | en | - |
| Μέγεθος | 4 pages | en |
| Τίτλος | Substrate rigidity dictates phenotype, survival, and mechanics of primary human osteosarcoma cells | en |
| Δημιουργός | Mylonaki Eleni | en |
| Δημιουργός | Μυλωνακη Ελενη | el |
| Δημιουργός | Dailiana, Z. | en |
| Δημιουργός | Trepat, X. | en |
| Δημιουργός | Lagoudakis Michael | en |
| Δημιουργός | Λαγουδακης Μιχαηλ | el |
| Περίληψη | Chemical cues and physical forces tightly control the milieu where a cell is born, survives, and finally dies. Of the physical forces, tissue rigidity is known to direct cell fate. Nonetheless, malignant tumors drastically alter the local physiological tissue rigidity. Our aim was to determine the functional changes of cells derived from an osteosarcoma, a very stiff malignant formation, to substrates of varying rigidity. Cells extracted from a human femoral osteosarcoma were exposed to collagen I-coated polyacrylamide substrates mimicking the rigidity of brain (1 kPa), connective tissue (7 kPa), and collagenous bone (55 kPa). Glass was used as a control substrate. Osteosarcoma cells occupied the smallest area and were rounder when on the compliant 1 kPa substrate compared to the stiffer substrates. Additionally, cell apoptosis, as assessed by Annexin V staining, and total death rate were significantly increased on the more compliant substrates of 1 and 7 kPa. Finally, as computed via a Fourier transform algorithm, cells exerted greater traction forces on the rigid 55 kPa substratum and less on the more compliant 7 and 1 kPa substrates. Taken together, these data suggest that osteosarcoma cells survive and function more efficiently on substrates mechanically closer to their native microenvironment. | en |
| Τύπος | Πλήρης Δημοσίευση σε Συνέδριο | el |
| Τύπος | Conference Full Paper | en |
| Άδεια Χρήσης | http://creativecommons.org/licenses/by/4.0/ | en |
| Ημερομηνία | 2015-11-13 | - |
| Ημερομηνία Δημοσίευσης | 2008 | - |
| Θεματική Κατηγορία | Bio-process engineering | en |
| Θεματική Κατηγορία | Bioprocess engineering | en |
| Θεματική Κατηγορία | biochemical engineering | en |
| Θεματική Κατηγορία | bio process engineering | en |
| Θεματική Κατηγορία | bioprocess engineering | en |
| Βιβλιογραφική Αναφορά | El. Mylona, Z. Dailiana, X. Trepat, and M. G. Lagoudakis. (2008, June). Substrate rigidity dictates phenotype, survival, and mechanics of primary human osteosarcoma cells. [Online]. Available: http://thales.iacm.forth.gr/~emilona/MylonaESBMEProceedings08.pdf | en |
Copyright © DIAS 2013
