Institutional Repository
Technical University of Crete
Technical University of Crete
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| URI | http://purl.tuc.gr/dl/dias/A807AD7B-4F26-4A45-B27C-46C8B54C5CFF | - |
| Identifier | http://thales.iacm.forth.gr/~emilona/MylonaESBMEProceedings08.pdf | - |
| Language | en | - |
| Extent | 4 pages | en |
| Title | Substrate rigidity dictates phenotype, survival, and mechanics of primary human osteosarcoma cells | en |
| Creator | Mylonaki Eleni | en |
| Creator | Μυλωνακη Ελενη | el |
| Creator | Dailiana, Z. | en |
| Creator | Trepat, X. | en |
| Creator | Lagoudakis Michael | en |
| Creator | Λαγουδακης Μιχαηλ | el |
| Content Summary | Chemical cues and physical forces tightly control the milieu where a cell is born, survives, and finally dies. Of the physical forces, tissue rigidity is known to direct cell fate. Nonetheless, malignant tumors drastically alter the local physiological tissue rigidity. Our aim was to determine the functional changes of cells derived from an osteosarcoma, a very stiff malignant formation, to substrates of varying rigidity. Cells extracted from a human femoral osteosarcoma were exposed to collagen I-coated polyacrylamide substrates mimicking the rigidity of brain (1 kPa), connective tissue (7 kPa), and collagenous bone (55 kPa). Glass was used as a control substrate. Osteosarcoma cells occupied the smallest area and were rounder when on the compliant 1 kPa substrate compared to the stiffer substrates. Additionally, cell apoptosis, as assessed by Annexin V staining, and total death rate were significantly increased on the more compliant substrates of 1 and 7 kPa. Finally, as computed via a Fourier transform algorithm, cells exerted greater traction forces on the rigid 55 kPa substratum and less on the more compliant 7 and 1 kPa substrates. Taken together, these data suggest that osteosarcoma cells survive and function more efficiently on substrates mechanically closer to their native microenvironment. | en |
| Type of Item | Πλήρης Δημοσίευση σε Συνέδριο | el |
| Type of Item | Conference Full Paper | en |
| License | http://creativecommons.org/licenses/by/4.0/ | en |
| Date of Item | 2015-11-13 | - |
| Date of Publication | 2008 | - |
| Subject | Bio-process engineering | en |
| Subject | Bioprocess engineering | en |
| Subject | biochemical engineering | en |
| Subject | bio process engineering | en |
| Subject | bioprocess engineering | en |
| Bibliographic Citation | El. Mylona, Z. Dailiana, X. Trepat, and M. G. Lagoudakis. (2008, June). Substrate rigidity dictates phenotype, survival, and mechanics of primary human osteosarcoma cells. [Online]. Available: http://thales.iacm.forth.gr/~emilona/MylonaESBMEProceedings08.pdf | en |
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