Energy audits and check of energy performances are more and more urgent for achieving energy saving in existing buildings. At the same time, European directives are imposing ever stricter restrictions on energy losses in buildings. In this context, the assessment of the thermal permeability (U-Value) of building components is a decisive step for energy diagnosis, in order to achieve effective interventions to improve it. In this diploma thesis measurements of the coefficient of thermal transmittance in external walls and windows were made in the building K1 of the Department of Environmental Engineering of the Technical University of Crete. A data logger, two temperature sensors and one heat flow sensor were used for the measurements. Then the experimental data were introduced into the building's simulation, which was based on its thermal insulation study. Comparisons of experimental measurements with the simulation data, and then the new energy consumptions calculated in heating and cooling. Finally, the rate of change in energy needs was calculated in relation to the original building. The results show that the experimental measurements are close to the theoretically calculated U-Value of the simulation for the walls which is not the case with windows.