Ιδρυματικό Αποθετήριο
Πολυτεχνείο Κρήτης
Πολυτεχνείο Κρήτης
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| URI | http://purl.tuc.gr/dl/dias/89FC39C3-64A9-4BF5-9EEE-9A18E29468F4 | - |
| Αναγνωριστικό | https://pubs.acs.org/doi/10.1021/acs.jproteome.6b00180 | - |
| Αναγνωριστικό | https://doi.org/10.1021/acs.jproteome.6b00180 | - |
| Γλώσσα | en | - |
| Μέγεθος | 13 pages | en |
| Τίτλος | Proteome changes during transition from human embryonic to vascular progenitor cells | en |
| Δημιουργός | Tsolis Konstantinos C. | en |
| Δημιουργός | Bagli Eleni | en |
| Δημιουργός | Kanaki Katerina | en |
| Δημιουργός | Zografou Sofia | en |
| Δημιουργός | Carpentier Sébastien Christian | en |
| Δημιουργός | Bei Aikaterini | en |
| Δημιουργός | Μπεη Αικατερινη | el |
| Δημιουργός | Christoforidis Savvas | en |
| Δημιουργός | Zervakis Michail | en |
| Δημιουργός | Ζερβακης Μιχαηλ | el |
| Δημιουργός | Murphy Carol | en |
| Δημιουργός | Fotsis Theodore | en |
| Δημιουργός | Economou, Anastassios | en |
| Εκδότης | American Chemical Society | en |
| Περίληψη | Human embryonic stem cells (hESCs) are promising in regenerative medicine (RM) due to their differentiation plasticity and proliferation potential. However, a major challenge in RM is the generation of a vascular system to support nutrient flow to newly synthesized tissues. Here we refined an existing method to generate tight vessels by differentiating hESCs in CD34+ vascular progenitor cells using chemically defined media and growth conditions. We selectively purified these cells from CD34- outgrowth populations also formed. To analyze these differentiation processes, we compared the proteomes of the hESCs with those of the CD34+ and CD34- populations using high resolution mass spectrometry, label-free quantification, and multivariate analysis. Eighteen protein markers validate the differentiated phenotypes in immunological assays; nine of these were also detected by proteomics and show statistically significant differential abundance. Another 225 proteins show differential abundance between the three cell types. Sixty-three of these have known functions in CD34+ and CD34- cells. CD34+ cells synthesize proteins implicated in endothelial cell differentiation and smooth muscle formation, which support the bipotent phenotype of these progenitor cells. CD34- cells are more heterogeneous synthesizing muscular/osteogenic/chondrogenic/adipogenic lineage markers. The remaining >150 differentially abundant proteins in CD34+ or CD34- cells raise testable hypotheses for future studies to probe vascular morphogenesis. | en |
| Τύπος | Peer-Reviewed Journal Publication | en |
| Τύπος | Δημοσίευση σε Περιοδικό με Κριτές | el |
| Άδεια Χρήσης | http://creativecommons.org/licenses/by/4.0/ | en |
| Ημερομηνία | 2018-10-11 | - |
| Ημερομηνία Δημοσίευσης | 2016 | - |
| Θεματική Κατηγορία | Angiogenesis | en |
| Θεματική Κατηγορία | CD34+ | en |
| Θεματική Κατηγορία | Comparative proteomics | en |
| Θεματική Κατηγορία | Endothelial progenitor cells | en |
| Θεματική Κατηγορία | hESCs | en |
| Θεματική Κατηγορία | Multivariate analysis | en |
| Θεματική Κατηγορία | PLS | en |
| Θεματική Κατηγορία | Vascular cell differentiation | en |
| Βιβλιογραφική Αναφορά | K. C. Tsolis, E. Bagli, K. Kanaki, S. Zografou, S. Carpentier, E. S. Bei, S. Christoforidis, M. Zervakis, C. Murphy, T. Fotsis and A. Economou, "Proteome changes during transition from human embryonic to vascular progenitor cells," J. Proteome Res., vol. 15, no. 6, pp. 1995-2007, Jun. 2016. doi: 10.1021/acs.jproteome.6b00180 | en |
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