Institutional Repository
Technical University of Crete
Technical University of Crete
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| URI | http://purl.tuc.gr/dl/dias/89FC39C3-64A9-4BF5-9EEE-9A18E29468F4 | - |
| Identifier | https://pubs.acs.org/doi/10.1021/acs.jproteome.6b00180 | - |
| Identifier | https://doi.org/10.1021/acs.jproteome.6b00180 | - |
| Language | en | - |
| Extent | 13 pages | en |
| Title | Proteome changes during transition from human embryonic to vascular progenitor cells | en |
| Creator | Tsolis Konstantinos C. | en |
| Creator | Bagli Eleni | en |
| Creator | Kanaki Katerina | en |
| Creator | Zografou Sofia | en |
| Creator | Carpentier Sébastien Christian | en |
| Creator | Bei Aikaterini | en |
| Creator | Μπεη Αικατερινη | el |
| Creator | Christoforidis Savvas | en |
| Creator | Zervakis Michail | en |
| Creator | Ζερβακης Μιχαηλ | el |
| Creator | Murphy Carol | en |
| Creator | Fotsis Theodore | en |
| Creator | Economou, Anastassios | en |
| Publisher | American Chemical Society | en |
| Content Summary | Human embryonic stem cells (hESCs) are promising in regenerative medicine (RM) due to their differentiation plasticity and proliferation potential. However, a major challenge in RM is the generation of a vascular system to support nutrient flow to newly synthesized tissues. Here we refined an existing method to generate tight vessels by differentiating hESCs in CD34+ vascular progenitor cells using chemically defined media and growth conditions. We selectively purified these cells from CD34- outgrowth populations also formed. To analyze these differentiation processes, we compared the proteomes of the hESCs with those of the CD34+ and CD34- populations using high resolution mass spectrometry, label-free quantification, and multivariate analysis. Eighteen protein markers validate the differentiated phenotypes in immunological assays; nine of these were also detected by proteomics and show statistically significant differential abundance. Another 225 proteins show differential abundance between the three cell types. Sixty-three of these have known functions in CD34+ and CD34- cells. CD34+ cells synthesize proteins implicated in endothelial cell differentiation and smooth muscle formation, which support the bipotent phenotype of these progenitor cells. CD34- cells are more heterogeneous synthesizing muscular/osteogenic/chondrogenic/adipogenic lineage markers. The remaining >150 differentially abundant proteins in CD34+ or CD34- cells raise testable hypotheses for future studies to probe vascular morphogenesis. | en |
| Type of Item | Peer-Reviewed Journal Publication | en |
| Type of Item | Δημοσίευση σε Περιοδικό με Κριτές | el |
| License | http://creativecommons.org/licenses/by/4.0/ | en |
| Date of Item | 2018-10-11 | - |
| Date of Publication | 2016 | - |
| Subject | Angiogenesis | en |
| Subject | CD34+ | en |
| Subject | Comparative proteomics | en |
| Subject | Endothelial progenitor cells | en |
| Subject | hESCs | en |
| Subject | Multivariate analysis | en |
| Subject | PLS | en |
| Subject | Vascular cell differentiation | en |
| Bibliographic Citation | K. C. Tsolis, E. Bagli, K. Kanaki, S. Zografou, S. Carpentier, E. S. Bei, S. Christoforidis, M. Zervakis, C. Murphy, T. Fotsis and A. Economou, "Proteome changes during transition from human embryonic to vascular progenitor cells," J. Proteome Res., vol. 15, no. 6, pp. 1995-2007, Jun. 2016. doi: 10.1021/acs.jproteome.6b00180 | en |
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