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Ability to produce surfactants and degrade hydrocarbons from selected bacterial strains

Frygana Sotiria

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Year 2022
Type of Item Diploma Work
Bibliographic Citation Sotiria Frygana, "Ability to produce surfactants and degrade hydrocarbons from selected bacterial strains ", Diploma Work, School of Chemical and Environmental Engineering, Technical University of Crete, Chania, Greece, 2022
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Oil is the most important source of energy on the production of which the world economy depends not only now but also in the future. Unfortunately, however, in recent years there have been recorded accidents both in the extraction and transport of oil through the water routes that have caused a huge ecological disaster.The present dissertation through the experimental part, that constitutes it simulates the bioenforcement of oil in seawater by microorganisms. The main purpose was the bacterial growth of Paracoccus, Alcanivorax venustensis and Alcanivorax borkumensis in hydrocarbon and oil substrates and thus their degradation. In order to properly measure the bacterial population growth, a UV spectrophotometer was used at 600nm. Other than the bacterial growth an extraction of the oil from the bacterium Paracoccus was also carried out to determine to what extent the oil has degraded. The same procedure was done with the bacteria Alcanivorax venustensis and Alcanivorax borkumensis with the difference that each of the two developed at two different temperatures, T=14oC and T=25oC. At the end of the extractions from the bacteria, the quantification of hydrocarbons, which are part of the oil and are examined, was done with the technique of gas chromatography and mass spectroscopy (GC/MS) to see under which conditions microorganisms grow best.It was observed that none of the above bacteria managed to degrade the polycyclic aromatic hydrocarbons (PAH) while to a large extent Alcanivorax venustensis has degraded alkanes in 25oC.

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