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Biomass and composition of microbial communities in conventional and organic greenhouses

Frantzeskou Maria

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Year 2022
Type of Item Diploma Work
Bibliographic Citation Maria Frantzeskou, "Biomass and composition of microbial communities in conventional and organic greenhouses", Diploma Work, School of Chemical and Environmental Engineering, Technical University of Crete, Chania, Greece, 2022
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In the recent years, the concern of many scientists and farmers has shifted to soil quality and fertility. Non- sustainable agriculture practises are being replaced by sustainable, environmentally friendly practices by shifting from conventional to organic farming. The purpose of this thesis is to find the impact of continuous cultivation om agricultural land has over the years in combination with the influence of temperature (4 oC, 20 oC, 30 oC, 40 oC) on organic matter and soil microbial communities. Samples from five different conventional and five organic greenhouses were used, with drastically different time span of usage. For the calculation of soil organic C were used chloroform fumigation and direct extraction (CFDE), DNA isolation and dry analysis of soil samples. Biodiversity was calculated for biological greenhouses using alpha-diversity indices: Chao1, Shannon and phylogenetic diversity. Lastly, the results were compared and presented with possible errors that occurred during the processing of the samples. Based on the results, it was observed that the greenhouses with the most fertile soil are organic, as the organic matter of conventional greenhouses was found to have low concentration of organic matter.

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