Ιδρυματικό Αποθετήριο
Πολυτεχνείο Κρήτης
Πολυτεχνείο Κρήτης
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| URI | http://purl.tuc.gr/dl/dias/237C89BB-CA9D-4EC7-8DB6-0640753E1C0E | - |
| Αναγνωριστικό | https://doi.org/10.1016/j.pestbp.2022.105058 | - |
| Αναγνωριστικό | https://www.sciencedirect.com/science/article/pii/S0048357522000256 | - |
| Γλώσσα | en | - |
| Μέγεθος | 11 pages | en |
| Τίτλος | Fungicide resistance frequencies of Botrytis cinerea greenhouse isolates and molecular detection of a novel SDHI resistance mutation | en |
| Δημιουργός | Malandrakis Anastasios | en |
| Δημιουργός | Μαλανδρακης Αναστασιος | el |
| Δημιουργός | Krasagakis Nikolaos | en |
| Δημιουργός | Kavroulakis, Nektarios | en |
| Δημιουργός | Ilias Aris | en |
| Δημιουργός | Tsagkarakou, Anastasia, 19..- | en |
| Δημιουργός | Vontas John | en |
| Δημιουργός | Markakis Emmanouil A. | en |
| Εκδότης | Elsevier | en |
| Περιγραφή | The research is financed by the project “Smart Diagnostic tools and database to support precision plant protection in horticultural crops in Crete” with acronym ‘SmartPP’, funded by the Crete Operational Program 2014-2020 and co-funded by the European Regional Development Fund (ERDF), under the Priority Axis “Enhancing the Competitiveness, Innovation, and Entrepreneurship of Crete”, Action 1.b.1: Demonstration - Experimental Development Projects, Promoting Research and Innovation in RIS3Crete. | en |
| Περίληψη | Sensitivity of B. cinerea to commonly used fungicides against Gray mold with emphasis to the newer quinone outside inhibitor (QoIs), and succinate dehydrogenase inhibitors (SDHIs) was assessed during a monitoring survey from vegetable greenhouses in four representative regions of Crete. 42% from a total of 168 isolates were simultaneously resistant to boscalid, fluopyram, pyraclostrobin and fenhexamid but not to fludioxonil making this phenylpyrrole fungicide an excellent anti-resistance antifungal agent. Isolates with double resistance to SDHIs and QoIs were found in very high frequencies indicating a selection towards double resistance due to the use of pyraclostrobin-boscalid mixtures. A number of sdhB resistance mutations (H272R, N230I and P225F/H) were found in isolates also carrying the G143A cytb resistance mutation in the above isolates. A novel sdhB point mutation (I274V) was identified for the first time in B. cinerea isolates collected from greenhouses with a fluopyram spray history with specific resistance to SDHIs. A PCR-RFLP diagnostic assay was developed for the detection of this mutation in the sdhB gene. Mutations P225F/H and I274V were found to be associated with fitness penalties in terms of mycelial growth, sporulation or pathogenicity. Results suggest that, in order to retain effective control of gray mold in Crete, appropriate anti-resistance strategies should be implemented taking into account the high double SDHI and QoI resistance frequencies. Additional studies for monitoring the already known and the new SDHI-resistance mutations, are necessary in order to hinder the further spread and establishment of single or double resistant isolates of B. cinerea detected in greenhouses in Crete. | en |
| Τύπος | Peer-Reviewed Journal Publication | en |
| Τύπος | Δημοσίευση σε Περιοδικό με Κριτές | el |
| Άδεια Χρήσης | http://creativecommons.org/licenses/by/4.0/ | en |
| Ημερομηνία | 2024-01-09 | - |
| Ημερομηνία Δημοσίευσης | 2022 | - |
| Θεματική Κατηγορία | Fludioxonil | en |
| Θεματική Κατηγορία | Strobilurins | en |
| Θεματική Κατηγορία | Carboxamides | en |
| Θεματική Κατηγορία | Double resistance | en |
| Θεματική Κατηγορία | I274V mutation | en |
| Θεματική Κατηγορία | Botryotinia fuckeliana | en |
| Βιβλιογραφική Αναφορά | A. A. Malandrakis, N. Krasagaki, N. Kavroulakis, A. Ilias, A. Tsagkarakou, J. Vontas, and E. Markakis, “Fungicide resistance frequencies of Botrytis cinerea greenhouse isolates and molecular detection of a novel SDHI resistance mutation,” Pestic. Biochem. Physiol., vol. 183, May 2022, doi: 10.1016/j.pestbp.2022.105058. | en |
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