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Zinc nanoparticles: mode of action and efficacy against boscalid-resistant Alternaria alternata isolates

Malandrakis Anastasios, Kavroulakis Nektarios, Chrysikopoulos Constantinos

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URIhttp://purl.tuc.gr/dl/dias/5519CEBA-ABDA-4074-9702-C4A1231754B4-
Identifierhttps://doi.org/10.1016/j.scitotenv.2022.154638-
Identifierhttps://www.sciencedirect.com/science/article/pii/S0048969722017314-
Languageen-
Extent11 pagesen
TitleZinc nanoparticles: mode of action and efficacy against boscalid-resistant Alternaria alternata isolatesen
CreatorMalandrakis Anastasiosen
CreatorΜαλανδρακης Αναστασιοςel
CreatorKavroulakis Nektariosen
CreatorChrysikopoulos Constantinosen
CreatorΧρυσικοπουλος Κωνσταντινοςel
PublisherElsevieren
Content SummaryThe antifungal potential of ZnO-NPs against Alternaria alternata isolates with reduced sensitivity to the succinate dehydrogenase inhibitor (SDHI) boscalid, resulting from target site modifications, was evaluated in vitro and in vivo. ZnO-NPs could effectively inhibit mycelial growth in a dose-dependent way in both boscalid (BOSC) sensitive (BOSC-S) and resistant (BOSC-R) isolates. The fungitoxic effect of ZnO-NPs against the pathogen was significantly enhanced when combined with boscalid compared to the individual treatments in all phenotype cases (BOSC-S/R) both in vitro and in vivo. Fungitoxic effect of ZnO-NPs could be, at least partly, attributed to zinc ion release as indicated by the positive correlation between sensitivities to the nanoparticles and their ionic counterpart ZnSO4 and the alleviation of the ZnO-NPs fungitoxic action in the presence of the strong chelating agent EDTA. The superior effectiveness of ZnO-NPs against A. alternata, compared to ZnSO4, could be due to nanoparticle properties interfering with cellular ion homeostasis mechanisms. The observed additive action of the oxidative phosphorylation-uncoupler fluazinam (FM) against all phenotypes indicates a possible role of ATP-dependent ion efflux mechanism in the mode of action of ZnO-NPs. A potential role of ROS production in the fungitoxic action of ZnO-NPs was evident by the additive/synergistic action of salicylhydroxamate (SHAM), which blocks the alternative oxidase antioxidant action. Mixture of ZnO-NPs and boscalid, resulting in a “capping” effect for the nanoparticles and significantly reducing their mean size, probably accounted for the synergistic effect of the mixture against both sensitive and resistant A. alternata isolates. Summarizing, results indicated that ZnO-NPs can be effectively used against A. alternata both alone or in combination with boscalid, providing an effective tool for combating SDHI-resistance and reducing the environmental fingerprint of synthetic fungicides.en
Type of ItemPeer-Reviewed Journal Publicationen
Type of ItemΔημοσίευση σε Περιοδικό με Κριτέςel
Licensehttp://creativecommons.org/licenses/by/4.0/en
Date of Item2023-12-22-
Date of Publication2022-
SubjectCarboxamidesen
SubjectNanofungicidesen
SubjectPlant pathogensen
SubjectZnO-NPsen
SubjectSynergismen
Bibliographic CitationA. A. Malandrakis, N. Kavroulakis, and C. V. Chrysikopoulos, “Zinc nanoparticles: mode of action and efficacy against boscalid-resistant Alternaria alternata isolates,” Sci. Total Environ., vol. 829, July 2022, doi: 10.1016/j.scitotenv.2022.154638.en

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